Effect of pentamidine on the growth of Escherichia coli.

نویسندگان

  • H AMOS
  • E VOLLMAYER
چکیده

Since Thrower and Valentine (1943) observed the trypanocidal and bacteriostatic action of propamidine, a number of investigators have studied the antimicrobial action of the aromatic diamidines. Kohn (1943) reported the inhibition of growth of Escherichia coli and Staphylococcus aureus by propamidine. This observation was extended to include Lactobacillus casei and Streptococcus lactis strain R by Snell (1944), who showed that polyamines added at the same time as propamidine prevented the inhibition of growth. Bichowsky-Slomnitzki (1948a, b) found that nucleic acids would reverse the bacteriostatic effect of pentamidine and stilbamidine on Escherichia coli and Staphylococcus aureus as effectively as polyamines. Bernheim (1943) investigated the effect of propamidine on resting cells of Escherichia coli and observed the reduction of oxygen utilization on the substrates alanine and arginine and on peptone in presence of the drug. Respiration on glucose and pyruvate was much less sensitive to propamidine. Blaschko and Duthie (1945) suggested the inhibition of amine oxidases as a point of possible metabolic interference by these compounds. The present study of the inhibition by pentamidine of the growth of Escherichia coli strain K-12 was undertaken as one phase of the investigation of the effect of this diamidine on bacteriophage production in K-12. The reversal of growth inhibition by L-glutamic or a-ketoglutaric acids and the interference by pentamidine with transaminations involving glutamic acid constitute the subject matter of this paper.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Antibacterial effects of aqueous and alcoholic extracts of Thyme on enterohemorrhagic Escherichia coli

Background: Because of increasing resistance to current antibiotics, many attempts have been made by the researchers to find new compounds of plant derivatives as substitute for non-effective antibiotics. This research was performed to study the antibacterial properties of aquatic and alcoholic extracts of thyme on the clinical and standard strains of enterohemorrhagic Escherichia coli. Materi...

متن کامل

بررسی اثر ترکیبی نانوذرات اکسید منیزیم و اکسید آهن بر روی رشد و مورفولوژی باکتری‌های اشریشیاکلی و استافیلوکوکو‌س اورئوس در آبمیوه

Introduction: Nanoparticles (NPs) are one of the antibacterial substances, among them nanoparticles type MgO and Fe2O3 are less toxic to mammalian cells. So, the aim of this study was investigation of combination effects of iron oxide and magnesium oxide nanoparticles on the growth of Staphylococcus aureus and Escherichia coli (E.coli) to achieve the optimum combination of nanoparticles inhibit...

متن کامل

Inhibitory effects of alcoholic extracts of thyme on verotoxin pruduction of entro heorrhagic Escherichia coli o157:h7

Entrohemorrhagic Escherichia coli is a human pathogen that causes hemorrhagic colitis due to its verotoxin. It is an agent with food poisoning importance; a significant number of cases subsequntly develop hemolitic uremic syndrome (HUS) which is probably the most common cause of renal failure in childeren and occasionally resulting in death. Inhibition of bacterial growth in food is the most im...

متن کامل

Inhibitory effects of alcoholic extracts of thyme on verotoxin pruduction of entro heorrhagic Escherichia coli o157:h7

Entrohemorrhagic Escherichia coli is a human pathogen that causes hemorrhagic colitis due to its verotoxin. It is an agent with food poisoning importance; a significant number of cases subsequntly develop hemolitic uremic syndrome (HUS) which is probably the most common cause of renal failure in childeren and occasionally resulting in death. Inhibition of bacterial growth in food is the most im...

متن کامل

The Over-Expression of Biologically Active Human Growth Hormone in a T5-Based System in Escherichia coli, Studying Temperature Effect

We studied the expression of human growth hormone (hGH) in E. coli under a bacteriophage T5-base promoter in a pQE30 expression vector. For an efficient expression of hGH cDNA, a number of codons at the hGH N-terminal coding region were altered based on the E. coli major codons. An over-expression of hGH in the bacteria, carrying the recombinant plasmids, was observed at 37°C in the presence of...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of bacteriology

دوره 73 2  شماره 

صفحات  -

تاریخ انتشار 1957